Detection and Quantitation of Norovirus Genome Using Real-Time RT-PCR
نویسندگان
چکیده
منابع مشابه
Evaluation of various real-time RT-PCR assays for the detection and quantitation of human norovirus.
Human noroviruses (NoVs) are the most common viruses causing acute gastroenteritis in humans. Performance characteristics of two commercial quantitative NoV RT-PCR assays, the Norovirus real-time RT-PCR Kit (AnDiaTec) and the Type I and Type II kits (Generon), and the international assay as selected by the CEN/TC/WG6/TAG4 group were evaluated for the specific detection and quantitation of 59 No...
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A single tube, fluorogenic probe-based, real-time quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assay was developed for detection and quantitation of bovine respiratory syncytial virus (BRSV) using BioRad's iCycler iQ. Real-time Q-RT-PCR was compared with quantitative competitive RT-PCR (QC-RT-PCR) and viral titers. Viral mRNA levels were measured in BRSV-infected bovi...
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Shellfish are recognized as a potential vehicle of viral diseases. The aim of the present study was to determine the ability of two real-time RT-PCR methods (an in-house method and a commercial kit) for detecting Norovirus (NoV) belonging to genogroups GI and GII in shellfish. The analyses were performed both on a Norovirus Reference Panel (NRP), consisting of synthetic RNA, and on naturally co...
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ژورنال
عنوان ژورنال: Japanese Journal of Food Microbiology
سال: 2011
ISSN: 1882-5982,1340-8267
DOI: 10.5803/jsfm.28.139